During his fight with Mosaic, Manikin sprouts tail and becomes more reptilian. With the long coat, Manikin looks almost exactly the way the Spider-Man villain The Lizard looked in his first appearance ("The Amazing Spider-Man" Vol. 1, #6, November 1963).
As Maggie considers the source of her powers, she jokingly suggests being bitten by a radioactive chameleon. This is a reference to Stan Lee's most famous creation, Spider-Man (bitten by a radioactive spider).
Although this animated film premiered in 2007, it was produced in 2006 according to the credits.