Extended Data Fig. 3: Vitamin E rescues TFH cell from ferroptosis induced by GPX4 deletion.
From: Selenium–GPX4 axis protects follicular helper T cells from ferroptosis
a–d, Cd28–/– mice were adoptively transferred of WT or T-KO OT-II cells and then intraperitoneally immunised with OVA in Alum. At day 10 post immunisation, recipient CD8+ T (a) and Foxp3+ TREG (b) cells and OT-II TREG (c) and TFR (d) cells in spleen were analysed by flow cytometry (n = 5). e, f, Cd28–/– mice were adoptively transferred OT-II cells from T-KO mice, subcutaneously immunised with NP-OVA in Alum and treated every 2 days with control or Vitamin E (100 mg/kg) from day 1–9 post immunisation. f, At day 10, frequencies and numbers of CXCR5+Bcl-6+ TFH in popliteal lymph nodes were analysed by flow cytometry (n = 5). Numbers indicate the frequency of cells in the gated region. Each dot represents one mouse. Data are presented as mean ± SEM and analysed by two-sided Student’s t-test. Data are representative of two experiments with at least five mice per group.