Extended Data Fig. 7: Ferrostatin-1 rescues TFH cells in mice immunised with NP-OVA.
From: Selenium–GPX4 axis protects follicular helper T cells from ferroptosis
a–d, Cd4-CreGpx4wt/wt (WT) and Cd4-CreGpx4wt/flox (T-HET) mice were subcutaneously immunised with NP-OVA in Alum. At day 14 post immunisation, the frequencies of CD3+ and CD4+ T cells (a), CD44, CD69, and Ki-67 expression by CD4+ T cells (b), frequencies of Foxp3+ TREG and Bcl-6+Foxp3+ TFR cells (c), and IFNγ, IL-4, and IL-17A production by CD44+CD25–CXCR5–PD-1– non-TFH cells (d) were analysed by flow cytometry (n = 5). e–g, As the schematic of experiment design (e), mice were adoptively transferred OT-II cells, intraperitoneally immunised with NP-OVA in Alum and treated daily with DMSO (control) or Ferrostatin-1 (10 mg/kg) from day 7–9 post immunisation. At day 10, frequencies of donor OT-II cells in total CD4+ T cells (f) and frequencies and numbers of TFH and non-TFH OT-II cells (g) in spleen were analysed by flow cytometry (n = 5). h–l, C57/BL6/J mice were intraperitoneally immunised with NP-OVA in Alum and treated every 2 days with DMSO (control) or Ferrostatin-1 (2 mg/kg) s.c. from day 5–13 post immunization. At day 14, CD44+Foxp3–CXCR5– non-TFH cells (i), Ki-67 expression by non-TFH cells (j), IFNγ, IL-4, and IL-17A production by non-TFH cells (k), Foxp3+ TREG cells, and Bcl-6+Foxp3+ TFR cells (l) in draining popliteal lymph nodes were analysed by flow cytometry (n = 6). Each dot represents one mouse. Data are presented as mean ± SEM and analysed by two-sided Student’s t-test. Data are representative of two experiments with at least four mice per group.