Extended Data Fig. 9: The gating strategy to analyse peripheral CD4+ T cell subsets in human blood.
From: Selenium–GPX4 axis protects follicular helper T cells from ferroptosis
Within viable CD3+CD8–CD4+ T cells, TREG cells were defined as CD25highCD127low cells, circulating TFH cells as CD25–/lowCD45RA–CXCR5+ cells, activated TFH cells as ICOShighPD-1high or PD-1high CCR7low TFH cells, activated non-TFH cells as CXCR5–ICOShighPD-1high cells, TH1 cells as CD25–/lowCD45RA–CXCR3+CCR6–CCR7lowCCR4– cells, TH2 cells as CD25–/lowCD45RA–CXCR3–CCR6–CCR7lowCCR4+ cells, and TH17 cells as CD25–/lowCD45RA–CXCR3–CCR6+CCR7–CCR4+ cells.