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The joint effect of PPARG upstream genetic variation in association with long-term persistent obesity: Tehran cardio-metabolic genetic study (TCGS)

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Abstract

Purpose

This study is the first study that aims to assess the association between SNPs located at the PPARG gene with long term persistent obesity. In this cohort association study, all adult individuals who had at least three consecutive phases of BMI (at least nine years) in Tehran genetic Cardio-metabolic Study (TCGS) were included.

Methods

Individuals who always had 30 ≤ BMI < 35 and individuals who always had 20 < BMI ≤ 25 were assigned to the long-term persistent obese group and persistent normal weight group, respectively. Other individuals were excluded from the study. We used four gamete rules to make SNP sets from correlated nearby SNPs and kernel machine regression to analyze the association between SNP sets and persistent obesity or normal weight.

Results

The normal group consisted of 1547 individuals with the mean age of 40 years, and the obese group consisted of 1676 individuals with mean age of 48 years. Two groups had a significant difference between all measured clinical characteristics at entry time. The kernel machine result shows that nine correlated SNPs located upstream of PPARG have a significant joint effect on persistence obesity.

Conclusion

This is the first study on the association between PPARG variants with persistent obesity. Three of the nine associated markers were reported in previous GWAS studies to be associated with related diseases. For the studied markers in the PPARG gene, the Iranian allele frequency was near the American and European populations.

Level III

Case–control analytic study.

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Data availability

In this study, the information of individuals who had participated in TLGS and TCGS were used.

Code availability

We used Haploview, R ( SKAT package), and Plink 2 software.

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Acknowledgements

The authors would like to express their gratitude to the patients participating in the Tehran lipid and glucose and Tehran Genetic Cardiometabolic studies. Also special thanks to the DeCODE genetic company for doing the genetic screening.

Funding

In this study, we used a data set that was funded by the Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences (Tehran, Iran).

Author information

Authors and Affiliations

  1. Cellular, and Molecular Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, 19195-4763, Tehran, Iran

    Niloufar Javanrouh Givi, Leila Najd Hassan Bonab, Asiyeh Zahedi, Bahareh Sedaghati-khayat, Mahdi Akbarzadeh & Maryam S. Daneshpour

  2. Obesity Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Maryam Barzin

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Corresponding author

Correspondence to Maryam S. Daneshpour.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures were following the ethical standards of the ethics committee on human subject research at Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences with the code of 13960449.

Informed consent

In this study, the information of individuals who had participated in TLGS and TCGS were used. All subjects signed a consent form at each visit.

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All subjects signed a consent form at each visit.

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The publication of this study has been approved by all co-authors, as well as by the responsible authorities at the institute.

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Javanrouh Givi, N., Najd Hassan Bonab, L., Barzin, M. et al. The joint effect of PPARG upstream genetic variation in association with long-term persistent obesity: Tehran cardio-metabolic genetic study (TCGS). Eat Weight Disord 26, 2325–2332 (2021). https://doi.org/10.1007/s40519-020-01063-7

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  • DOI: https://doi.org/10.1007/s40519-020-01063-7

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