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Hi everyone:
I met a problem when I run the DUTest as below, so I find my peaks.seurat@tools$Sierra doesn't have any thing except the rownames.
res.table = DUTest(peaks.seurat,
-
population.1 = "W-P", -
population.2 = "L-P", -
exp.thresh = 0.1, -
#feature.type = c("UTR3", "exon") -
)
[1] "0 expressed peaks in feature types UTR3, exon"
[1] "0 genes detected with multiple peak sites expressed"
[1] "0 individual peak sites to test"
Error in [.data.frame(Tool(apa.seurat.object, "Sierra"), , c("Gene_name", :
选择了未定义的列
And my annotation_peak.txt as below:
I have no idea why it lost the information of Sierra dataframe when combine the seruat object and annotated peaks. Can anyone help me?
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