8000 Convert vector_type==unspecified to ssAAV or scAAV by heuristic, or remove the option · Issue #44 · formbio/laava · GitHub
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Convert vector_type==unspecified to ssAAV or scAAV by heuristic, or remove the option #44
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@etal

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@etal

In milestone v3.0, the report will depend on the designed vector type being known -- either ssAAV or scAAV. Currently, the input field vector_type allows a third option, "unspecified".

Consider a heuristic to convert "unspecified" to ss or sc based on the contents of the processed data, just before reporting:

  • Majority frequency of "full" subtypes with ssAAV vs. scAAV polarities/strandedness
  • Median read length -- we expect ssAAV to be around 4.7 kilobases and scAAV around half that; consider a cutoff around 2.5kb if the majority-frequency heuristic isn't enough.

Alternatively, just remove the "unspecified" option from the vector_type field if we expect users to always know the designed vector type and heuristic auto-detection is either unhelpful or unreliable.

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