8000 --protein-name-separator drops proteins · Issue #380 · percolator/percolator · GitHub
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--protein-name-separator drops proteins #380

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stavis1 opened this issue Jul 10, 2024 · 0 comments
Open

--protein-name-separator drops proteins #380

stavis1 opened this issue Jul 10, 2024 · 0 comments

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@stavis1
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stavis1 commented Jul 10, 2024

Hello,

I am working with rel-3-07-01 and I am running into a problem with the -K/--protein-name-separator parameter. When used only the first protein in the list of proteins that a PSM matches to shows up.

Specifically, when I run:
percolator -U -m tab_sep.out QE3_RP180_4ug_Exp13_r2_t3.pin
the output for an example PSM looks like:
QE3_RP180_4ug_Exp13_r2_t3_76331_2_1 2.78719 0.00356664 4.73844e-08 R.CTEECPFGALDDDEK.G bin_049_000000000018_14 bin_050_000000000187_2 bin_064_000000000037_3

Whereas when I run:
percolator -U -K , -m comma_sep.out QE3_RP180_4ug_Exp13_r2_t3.pin
The same PSM looks like:
QE3_RP180_4ug_Exp13_r2_t3_76331_2_1 2.78719 0.00356664 4.73844e-08 R.CTEECPFGALDDDEK.G bin_049_000000000018_14
when instead I would expect a comma separated list of protein IDs.

This issue is slightly different from similar issues that have arisen before, e.g. #343, in that the protein name column is not completely empty.

I have tested all of the 3.6 releases and the same problem occurs in all of them. I am running Ubuntu and have tried both the standalone executable version (percolator-noxml-ubuntu-portable.zip) and the deb version (percolator-noxml-v3-07-linux-amd64.deb). I have also tried both -K and --protein-name-separator as well as several different separator characters.

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