Genome Inspector 1.0 is a graphical user interface (GUI) application for performing various DNA sequence analyses. It is designed to be user-friendly and accessible for researchers, students, and anyone interested in molecular biology. The application leverages the Tkinter library for the GUI and Biopython for powerful sequence analysis capabilities.
- DNA to RNA Conversion: Convert a DNA sequence to its RNA counterpart.
- DNA Complement: Generate the complementary DNA sequence.
- DNA to Amino Acid: Translate a DNA sequence to its corresponding amino acid sequence (one-letter and three-letter codes).
- Translate Reading Frame: Translate the DNA sequence starting from a specified reading frame.
- Reverse Complement: Generate the reverse complement of the DNA sequence.
- Find Restriction Sites: Identify the locations of specified restriction enzyme sites within the DNA sequence.
- Find ORFs: Detect open reading frames (ORFs) within the DNA sequence.
- Count Nucleotide Frequencies: Count occurrences of each nucleotide (A, T, G, C) in the DNA sequence.
- GC Content: Calculate the GC content percentage in the DNA sequence.
- Melting Temperature: Compute the melting temperature of the DNA sequence.
- Annealing Temperature: Estimate the annealing temperature, typically 5°C lower than the melting temperature.
- Quick Analysis: Perform a quick summary analysis, including base count, GC content, melting temperature, annealing temperature, and nucleotide frequencies.
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Clone the repository:
git clone https://github.com/AtharvaTilewale/genome-inspector.git cd genome-inspector -
Install required dependencies:
- Ensure you have Python installed (version 3.6 or higher).
- Install the required Python packages using pip:
pip install biopython pip install tkinter
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Installation
chmod +x setup ./setup
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Run the program
geneinspect
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Launch the application: Run the Python script to start the Genome Inspector GUI.
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Input DNA Sequence:
- You can manually enter a DNA sequence in the input field.
- Alternatively, use the "Browse File" button to upload a sequence file (FASTA, FASTQ, or plain text).
- Select Analysis Option:
- Choose from various analysis options by clicking the respective buttons on the home screen.
- For restriction site analysis, you will be prompted to enter the enzyme site.
- View Results:
- The results of the selected analysis will be displayed in the text area within the application.
- You can save the results to a text file using the "Save Results" button.
Contributions are welcome! Please follow these steps:
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Fork the repository.
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Create a new branch for your feature or bugfix.
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Commit your changes with a descriptive message.
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Push your branch to your fork.
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Open a pull request detailing your changes.
This project is licensed under the MIT License. See the LICENSE file for more details.
If you have any questions, suggestions, or feedback, feel free to open an issue or contact the project maintainers.
Genome Inspector 1.0 © 2024 Atharva Tilewale