Authors: Jakob Jung, Patrick Pfau, Lars Barquist
Date: 03-05-2022
This is the github page of the MASON webserver. MASON (Make AntiSense Oligos Now) is a user-friendly web-tool that can be used to design ASO sequences for any gene in any bacterium of interest. MASON can be accessed via https://www.helmholtz-hiri.de/en/datasets/mason/.
In addition to generating ASO sequences able to bind a target gene, it calculates sequence attributes such as the melting temperature of ASO-RNA interaction and predicts possible off-targets within the targeted microbes.
The code of the web-server, and explanations of how to run the server on a local computer can be found in the subfolder browser. The code is published under the MIT license.