Calculate the manimum free energy for mIR duplex ends with 4bp Please follow the below step to obtain results:
Please provide input file with two columns in which first column for 5p and 3p in the second from the miRNA duplex sequence. test inputfile is provided with code It's required pairwise2 python package (pip/pip3 install pairwise2)
./MFEcalc.sh input_file