For Agilent NGS Workstation Option B
This document describes how to set up the automated transfer protocols for the Agilent NGS Workstation created at NGI Stockholm.
- Agilent NGS Workstation
⚠️ Option B only - Consumables
- Eppendorf twin.tec 96 PCR plate (Eppendorf, cat# 0030 128.672 (int); 951020460 (US))
- Nunc deepwell 1.3 mL plate (Thermo Scientific, cat# 260251)
- ABgene 2.2 mL storage plate Mk.II (Thermo Scientific, cat# AB-0933)
- Deepwell plate adaptor on position 6 (Agilent Technologies, cat# G5498B#012) and supporting device file
- Labware definitions*
- Liquid classes definition*
* provided in all_labware_liquids.vzp
transfer.pro
transfer_dilution.pro
transfer_form.VWForm
transfer_lib.js
all_labware_liquids.vzp
resources/1313497192_media_controls_light_pause.png
resources/1313497517_media_controls_light_play.png
resources/clear_inventory.bat
resources/clear_inventory.sql
Clone into https://github.com/ngi-automation/transfer-protocols.git from the Protocol Files directory:
cd "C:\VWorks Workspace\Protocol Files"
git clone https://github.com/ngi-automation/transfer-protocols.gitAlternatively, download the compressed folder from:
https://github.com/ngi-automation/transfer-protocols/archive/master.zip
and extract to C:\VWorks Workspace\Protocol Files. Rename the resulting transfer-protocols-master folder to transfer-protocols. The path to the folder containing the extracted files should then be C:\VWorks Workspace\Protocol Files\transfer-protocols.
Use the import feature in VWorks from File › Importin the toolbar and select the all_labware_liquids.vzp file included. See the VWorks Knowledge Base for more information.
Device files and profiles are system specific and will not be provided. The following Bravo configuration is used in the transfer protocols:
| Position | Type | Part# |
|---|---|---|
| 1, 2, 8 | Deck Platepad | G5498b#004 |
| 3 | Mirrored Barcode Reader | G5498B#031 |
| 4, 6 | Peltier Thermal Station (Inheco) | G5498b#021 |
| 5 | Orbital Shaking Station | G5498b#033 |
| 7 | Magnetic Bead Accessory | G5498b#008 |
| 9 | Thermal Station (ThermoCube) | G5498b#036/7/8 |
For reference see Agilent's accessories catalog for the Bravo.
Each .pro file must have the correct device file set. See the VWorks Knowledge Base for more information on how to select the device file.
See the NGI Stockholm/SciLifeLab Standard Operating Procedure.
Licensed under the Apache License, Version 2.0 (the "License"); you 5D77 may not use this file except in compliance with the License. You may obtain a copy of the License at
http://www.apache.org/licenses/LICENSE-2.0
Unless required by applicable law or agreed to in writing, software distributed under the License is distributed on an "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the License for the specific language governing permissions and limitations under the License.
The full license can also be found in the file LICENSE and must included when redistributing the software.
If this method is used to generate results for publication we ask that you include a reference to this repository, something like:
Automation protocols made available by NGI Sweden at https://github.com/ngi-automation/transfer-protocols
VWorks Automation Control, Bravo and other things relating to the Agilent NGS Workstation are trademarks owned by Agilent Technologies, Inc. (Santa Clara, CA 95052-8058, US).
National Genomics Infrastructure at SciLifeLab
joel.gruselius@scilifelab.se
October 2015