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Eutherian Corticogenesis

This is a repository for the analysis performed in the paper Eutherian Enhancers Important in Cortical Development

Tissue Extraction

Published Data

Cell Ranger Parameters

Mouse

--transcriptome=/fast/users/newmana_c/work/10X/refdata-gex-mm10-2020-A
--sample=m_mouse
--expect-cells=10000
--jobmode=slurm
--maxjobs=100
--jobinterval=1000
--include-introns

Opossum

Opossum transcriptome was generated....

Hi

--transcriptome=/fast/users/newmana_c/work/10X/Monodelphis.domestica_genome
--expect-cells=10000
--jobmode=slurm
--maxjobs=100
--jobinterval=1000
--include-introns

Analysis in R

Dependencies

Normalization & Integration

Integration, normalization and scaling of data output by cellranger runs was integrated using the opossum_seurat_E14_E16_E18_.Rmd script.

Cells from all samples belong to the pyramidal lineage are then subset and saved in a file 'sc_seurat.PN.sct.rds'.

Plotting & Differential Expression

Gene Ontology of Species Specific Differences by Cluster

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