8000 GitHub - sanvva/OM-HiC-scaffolding: Workflows for correcting and scaffolding long-read (PacBio, nanopore) genome assemblies using optical mapping and/or Dovetail Hi-C data
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Workflows for correcting and scaffolding long-read (PacBio, nanopore) genome assemblies using optical mapping and/or Dovetail Hi-C data

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##OM-HiC-scaffolding

Here the scripts are used in the paper "Improving and correcting the contiguity of long-read genome assemblies of three plant species using optical mapping and chromatin conformation capture data". http://genome.cshlp.org/content/27/5/778

#Description These scripts are used to do genome assembly scaffolding in three ways:

  1. scaffolding using optical consensus maps and PacBio read assembly contigs from Falcon and PBcR

  2. scaffolding using Dovetail Hi-C reads and PacBio read assembly contigs from Falcon and PBcR

  3. scaffolding using optical consensus maps, Dovetail Hi-C reads and PacBio read assembly contigs from Falcon and PBcR

#System requirements

Perl(version >5.10); Bioperl

IrysSolve scripts http://www.bnxinstall.com/scripts/v3692/

RefAligner http://www.bnxinstall.com/RefalignerAssembler/Linux/SSE/

BLAST (makeblastdb, blastn)

HiRise (https://github.com/DovetailGenomics/HiRise_July2015_GR)

####requirements for HiRise  
Python 3

BWA (version: 0.7.15-r1140)

samtools (version:1.2)

samblaster (https://github.com/GregoryFaust/samblaster)

Meraculous (http://downloads.sourceforge.net/project/meraculous20/release-2.0.5.tgz)

#Usage Please read the "README.md" and "run.sh" in each scaffolding worflow

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Workflows for correcting and scaffolding long-read (PacBio, nanopore) genome assemblies using optical mapping and/or Dovetail Hi-C data

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