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Genome Assembly Annotation Service (GAAS)

Contains development done in the GAA (Genome Assembly Annotation) Service.

Annotation directory contains development related to annotation side of the service.

Shorcuts:

Assembly directory contains development related to assembly side of the service.

Shorcuts:


Installation

  • Clone and install GAAS

To use the tools in this repository, clone the directory and run make install to update some paths:

git clone https://github.com/NBISweden/GAAS.git
cd GAAS
  • A) For the use through env

    • A.1) Updates paths in the environment profiles to point to the correct GAAS repository location
    make install   
    
    • A.2) Dependencies

      • You might check that all dependencies are filled up. Depending the scripts you want to use, all dependencies are not required.
      make check
      
      • install the missing dependencies of your choice
        Bioperl and perl libraries with cpanm
        cpanm bioperl
        cpanm Clone
        cpanm Moose 
        cpanm Graph::Directed
        cpanm LWP::UserAgent
        cpanm Statistics::R
        cpanm JSON
        cpanm Sort::Naturally
        
    • A.3) Load the correct profiles (add NBIS libs and tools to the PATH)
      Three profiles are available to setup the necessary environment variables to use the scripts:

      • By default:
      source profiles/activate_env
      
      • If you are on Rackham:
      source profiles/activate_rackham_env
      
      • If you are on NBIS's servers:
      source profiles/activate_nbis_env
      
    • A.3) To get out of the nbis environment and restore your previous environment type

    deactivate
    
  • B) For a permanent use

    • B.1) You might check that all dependencies are filled up. Depending the scripts you want to use, all dependencies are not required.
    make check
    
    • B.2) Add the path to the BILS perl library as well as the bin folder containing all tools. You can add in you ~/.bashrc or ~/.profile file.
    export PERL5LIB=$PERL5LIB:/pathTo/GAAS/annotation
    export PATH=${PATH}:/pathTo/GAAS/annotation/Tools/bin
    

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